RonSean Posted February 27, 2014 Share Posted February 27, 2014 I am currently trying to express a toxic Fab protein using e.coli. Unfortunately, I have to use this expression vector. I can successfully make the Fab protein at the 2ml scale but have not been able to express at larger 50-100ml scale. Details: pComb vector, SB media, Carbenicillan, with and without glucose, IPTG expression system (non T7 driven) and an e.coli strain that has the laq Iq gene to over express the lac repressor. I am a molecular biologist and am new to protein expression in e.coli. Any advice would be greatly appreciated. Link to comment Share on other sites More sharing options...
AlanaC Posted March 7, 2014 Share Posted March 7, 2014 Have you tried altering time/temperature of induction to optimise it?Also sometimes the concentration of inducer can affect things along with the type of media used. Link to comment Share on other sites More sharing options...
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