Guest torontostudent Posted June 8, 2004 Share Posted June 8, 2004 I have been using a method to calculate the activity of glucose-6-phosohate dehydrogenase. I ran into a problem because my final activities are very low compared to results previously obtained. I think I'm just doing the calculations wrong. In a nutshell, I make up reactions in cuvettes and I measure the rates and I have to subtract certain rates from other rates. Then I'm supposed to use the "net optical density" from my subtracted rates. How do I do calculate net optical density from a rate? Link to comment Share on other sites More sharing options...
ATCC-6538 Posted June 16, 2004 Share Posted June 16, 2004 You measure the appearance of NADPH. Your blanc is: all components except your sample (enzyme), in some cases it is recomemnded to measure blanc, where substrate (G-6-P) is missing (NADP/NADPH is a cosubstrate in many reactions). So, where is the problem? I need more info! Link to comment Share on other sites More sharing options...
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