daisy Posted June 11, 2003 Share Posted June 11, 2003 Anyone out there with any experience? I'd be grateful for a link to a good trouble-shooting guide. I've been embarked on this for a few weeks but never having done it before I'm not too aware of the pitfalls. Any good websites gratefully recieved!! Link to comment Share on other sites More sharing options...
Guest cjdf Posted July 9, 2003 Share Posted July 9, 2003 What exactly are you doing in relation to protein expression in bacteria...? Link to comment Share on other sites More sharing options...
daisy Posted July 9, 2003 Author Share Posted July 9, 2003 I've moved on a bit now....any tips on site directed mutagenesis?? I can't get it to work. I've tried changing template amounts, DMSO, used a gradient block to vary annealing temp, used a different clone, tried a kit, tried the standard lab protocol..... Any magic wands out there? Link to comment Share on other sites More sharing options...
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