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Typical molar ratio of lipid to receptor in an overexpressing cell line?


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#1 DrJake

DrJake

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Posted 8 February 2017 - 04:05 PM

I recently tested some synthetic proteoliposomes made via in vitro transcription/translation of a GPCR in a liposome matrix. The manufacturer gives an approximate molar ratio of lipid:GPCR of 150:1, which seems rather high. I'm curious whether anyone has an idea of the molar ratio (or range) you might expect in proteoliposomes made from recombinant GPCR-overexpressing cell lines (either stable or inducible). 

 

First post here, thanks for any responses.


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