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hasmatbanu buchad

RNA RNA EMSA

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Hello

I want details regarding RNA-RNA EMSA. I require your valuable suggestion for my problem with experiment.

I used two RNA for gel shift assay one of which is labelled with Digoxygenin. I get some shift in EMSA. I also perform the same thing with unlabelled RNA and perform EMSA assay as previous one and stained my gel with Ethidium Bromide but not able to detect any shift.
I am not able to conclude anything out of it.
Kindly suggest regarding
Waiting for your reply
Regards,
Hasmatbanu Buchad
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