Gamewizard Posted July 17, 2015 Share Posted July 17, 2015 Hi quick question What would be the bacterial concentration at 0.6 OD ? and how would you write that as ? thank you Link to comment Share on other sites More sharing options...
Enthalpy Posted July 17, 2015 Share Posted July 17, 2015 Hi, OD=0.6 only means that the power of light passing through is divided by 100.6. You would need the light's path length and some attenuation (possibly by diffusion) properties of these specific bacteria to deduce a concentration, provided nothing else attenuates. Link to comment Share on other sites More sharing options...
CharonY Posted July 17, 2015 Share Posted July 17, 2015 That is absolutely dependent on your bacterium and the medium you are using. You will either have to count the cells or plate them (life titer) for a given OD range and plot a calibration curve. Link to comment Share on other sites More sharing options...
Gamewizard Posted July 18, 2015 Author Share Posted July 18, 2015 Thank you for your replies, I will have to do plate counts for it Link to comment Share on other sites More sharing options...
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