TUDI Posted March 1, 2015 Share Posted March 1, 2015 I need to set-up an experiment using E.coli which I can follow for couple of weeks. The lecture is bioprocess engineering. We have very limited equipments in the lab so the growth must be either in flasks or petri dishes. Any suggestion for such an experiment ? What kind of parameters can be followed and what kinds of optimizations can be made? Link to comment Share on other sites More sharing options...
John Cuthber Posted March 1, 2015 Share Posted March 1, 2015 A wise man once pointed out to me that you should never use e coli in an experiment where you could use brewing yeast. Yeast colonies smell better. Also, they are not pathogens. Link to comment Share on other sites More sharing options...
TUDI Posted March 2, 2015 Author Share Posted March 2, 2015 A wise man once pointed out to me that you should never use e coli in an experiment where you could use brewing yeast. Yeast colonies smell better. Also, they are not pathogens. Ok thanks So can you suggest me a simple set up for this experiment and how can I continue this process for weeks? Link to comment Share on other sites More sharing options...
CharonY Posted March 2, 2015 Share Posted March 2, 2015 How about getting a home brewing kit? 1 Link to comment Share on other sites More sharing options...
John Cuthber Posted March 2, 2015 Share Posted March 2, 2015 A few home-brew kits at different temperatures. 1 Link to comment Share on other sites More sharing options...
CharonY Posted March 2, 2015 Share Posted March 2, 2015 Good idea, or different yeast varieties with various optimum temps. 1 Link to comment Share on other sites More sharing options...
John Cuthber Posted March 2, 2015 Share Posted March 2, 2015 Or a few different sugar concentrations or pH levels. 1 Link to comment Share on other sites More sharing options...
CharonY Posted March 2, 2015 Share Posted March 2, 2015 And do not forget the subsequent taste analysis! 1 Link to comment Share on other sites More sharing options...
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