jmcnell Posted April 4, 2014 Share Posted April 4, 2014 I've been up all day and am kind of frustrated I can't figure this out. I don't want to mess it up before lab and ruin my entire protocol. I need to make a 2mg/100µl solution with 1000mg NaCl, thus I need to deduce the volume needed to mix the NaCl with to give this ratio of 2mg/100µl I thought the formula was (m1)(v1)=(m2)(v2) but that doesn't seem right when I did the calculation. can some please point me into the right direction for this calculation. I've been up all day doing exams and I am just whiped out right now.. Link to comment Share on other sites More sharing options...
studiot Posted April 4, 2014 Share Posted April 4, 2014 Well 1000mg is 500 times as much as 2mg So you will need 500 times as much volume as for 2mg ie 500 x 100 microlitres. Link to comment Share on other sites More sharing options...
jmcnell Posted April 5, 2014 Author Share Posted April 5, 2014 50 mL or 50,000 microliters correct? Thanks!! Link to comment Share on other sites More sharing options...
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