Ugo51 Posted December 12, 2013 Share Posted December 12, 2013 Hi everyone, how would you expect hepatocytes to take up sodium acetate from the culture medium? Cheers Link to comment Share on other sites More sharing options...
Iota Posted December 12, 2013 Share Posted December 12, 2013 Tell us what you think first Link to comment Share on other sites More sharing options...
Ugo51 Posted December 13, 2013 Author Share Posted December 13, 2013 the thing is that I don't really think much... Sodium acetate should slightly dissolve in the cellular medium (pKa in water is about 4, right?). I would then expect the acetate to diffuse though the membrane. If all the acetate was to be utilyzed for FA synthesis (i.e. converted to Acyl-CoA) this would skew the equilibrium of the solution, more NaAcetate will dissolve and diffuse into the cells and so on until no acetate is left in the medium. Anyway, beside the fact I don't think this is going to happen quite quickly, I have no idea what will happen in the meantime to the undissociated NaAcetate. I know there is a range of ion/solute symporter/transporter on the plasma membrane, but I just have no idea which are expressed on hepatocytes and to what extent they partecipate in the process of acetate uptake. Link to comment Share on other sites More sharing options...
Craer Posted December 14, 2013 Share Posted December 14, 2013 (edited) Do you think an additional factor could increase or affect this acetate uptake? Edited December 14, 2013 by Craer Link to comment Share on other sites More sharing options...
Ugo51 Posted December 14, 2013 Author Share Posted December 14, 2013 Sure. What I'm trying to understand is what mediates such transport Link to comment Share on other sites More sharing options...
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