mohamed samir Posted March 11, 2013 Share Posted March 11, 2013 Dear ALl,Alittle bit stricky for me to ask question like this.Iam going to perform an experiment on a co- culture consists of both human A549 pneumocyte II cells and murine macrophage J774 cell line to test the effect of miRNAs on influenza virus replication .I have no protocol for this and iam confused really whether I will be able to blend two cell line from different host. However, the media in both cells are differ , is this will pe appropriate medium for both cells ?Should I used human macrophage cell line to be compatable with the A549 cell as it is from human ?Dpepnding on which criteria, will our biculture succeed ? I can summarize the questionCan I culture two differenrt cell line ( for example, A549 and J774 ) at the time both of them was cultured in different medium ?Can I culture two differenrt cell line ( for example, A549 and J774 ) that isolated from two different animal host or human ? Thanks Link to comment Share on other sites More sharing options...
Gaylord Posted March 15, 2013 Share Posted March 15, 2013 What do you hope to see? Link to comment Share on other sites More sharing options...
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