Just a little assurance needed please :)

[ozric]

Hi there. I've been doing some biochemical tests on unknown microorganisms. They're from a UK kitchen, so nothing too exotic!

 

I was just wondering if I have sufficient evidence to convict;

 

1) a microorganism as Bacillus spp with the following:

 

Gram: positive rods, spores visible

Catalase: positive

Oxidase: negative

MacConkey: negative

 

2) a microorganism as Pseudomonas spp with the following:

 

Gram: negative rods

Catalase: positive

Oxidase: positive

MacConkey: negative

Urease: negative

 

3) a microorganisms as Klebsiella spp with the following:

 

Gram: negative rods

Catalase: positive

Oxidase: negative

MacConkey: positive

Urease: positive

Indole: negative

ONPG: positive

 

and 4) a microorganisms as E. coli with the following:

 

Gram: negative rods

Catalase: positive

Oxidase: negative

MacConkey: positive

Urease: negative

Indole: positive

ONPG: positive

 

Thanks in advance!

 

:)

[jorge1907]

Probably all good bets but not enough info to be assured.

#1 - might also be Geobacillus or Brevibacterium

#2 - could be alot of Gram neg nonfermenters

#3 - good bet but could be another enteric - e.g. Enterobacter agglomerans

#4 - real gooc bet - but could be E. agglomerans and some of the other Escherichia sp.

 

 

if you have singe islates - describe as "sp." - "spp." is plural.

[ozric]

Probably all good bets but not enough info to be assured.

#1 - might also be Geobacillus or Brevibacterium

#2 - could be alot of Gram neg nonfermenters

#3 - good bet but could be another enteric - e.g. Enterobacter agglomerans

#4 - real gooc bet - but could be E. agglomerans and some of the other Escherichia sp.

 

 

if you have singe islates - describe as "sp." - "spp." is plural.

 

Hi, thanks for your suggestions.

 

Agreed, I may need some more results to positively identify. For the Pseudomonas, as it is from the kitchen, from tap water, I thought this was the most likely organism.

 

For the others, yes there are a few alternatives. I'll have to look further into other tests I can do.

 

Thanks again

[JorgeLobo]

Bet you're right sbout the pseudomonad - were colony morphology, smelll and pigmentation consistent with P. aeruginosa?

[ozric]

Bet you're right sbout the pseudomonad - were colony morphology, smelll and pigmentation consistent with P. aeruginosa?

 

Hi there.

 

Colonies were a creamy white colour, about 1-2 mm in diameter on blood agar. To be honest, I can't remember what it smelt like and didn't note that down.

[JorgeLobo]

So P. aeruginosa is very unlikely.

[ozric]

 

This is what they looked like (picture from google images).

Edited April 17, 2012 by ozric