preventing clotting of blood

[blackfang320]

hi guys i was reading around and found that edta can prevent clotting of blood. i have a glass vial that holds 6mm and i want to fill it with human blood but i do not want it to clot so i got tertasodium edta hoping to prevent it from clotting . can anyone tell me if it will work or how much edta i should add to keep the blood from clotting as long as possible? thanks for the help

[rktpro]

i have a glass vial that holds 6mm and i want to fill it with human blood

 

Thou shall not take thy neighbor's blood.

Edited April 6, 2012 by rktpro

[blackfang320]

its not my neighbor's blood its mine

[ecoli]

EDTA or citrate are common anticoagulates. I'm not sure what the correct concentrations are, but I'm sure that data can be found in medical journals (a lot of studies use anticoagulents)

[blackfang320]

thank you so much do you know where i might be able to find one of thoes journals?

[CharonY]

The vials that we use contain 1.8 mg EDTA per ml blood. Note that in commercial tubes EDTA is sprayed to the walls, which facilitates easy mixing. If you just pipette it in, you have to be quick and ensure thorough mixing.

Edited April 7, 2012 by CharonY