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Concentration help

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My SDS separating gel contains Tris/SDS and im trying to calculate the conc of this in the gel....


The tris/sds stock solution was made initially from:


6.05 g Tris


20ml of 20% SDS


80 ml water to give a total volume of 100ml. So Tris/SDS was made in this solution.


I want to calculate to concentration of Tris/SDS in this solution, so do i have to calculate the tris and SDS concs seperatly or together,,,if you get what i mean.


I hope this is clear, any help will be much obliged

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