whusean 0 Posted January 31, 2012 My SDS separating gel contains Tris/SDS and im trying to calculate the conc of this in the gel.... The tris/sds stock solution was made initially from: 6.05 g Tris 20ml of 20% SDS 80 ml water to give a total volume of 100ml. So Tris/SDS was made in this solution. I want to calculate to concentration of Tris/SDS in this solution, so do i have to calculate the tris and SDS concs seperatly or together,,,if you get what i mean. I hope this is clear, any help will be much obliged 0 Share this post Link to post Share on other sites