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Dima

SDS-PAGE

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....separates proteins by size. But, technically does the charge affect the separation AT ALL or is negligible? I don't think anything should be negligible in science do you lol?

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I wouldn't say charge is negligible. Granted, size of protein has a larger impact than charge, but its the mass/charge ratio which is taken into consideration.

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yes, but the argument is that the SDS coats the proteins giving them all an equal charge supposedly. Is it true?

Edited by Dima

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