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eukaryotic protein


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Hi Ghaz,

 

unfortunately there are many reasons that can justify the lack of activity of an eukaryotic protein produced by bacteria. Some of the most recurrent problems are:

1. Absence of post-translational modifications in the producing cell

2. Absence of chaperones suitable for a proper folding of the protein

3. Non-specific interactions with host proteins and/or other molecules

4. Structure alterations due to lab manipulations during the isolation and purification of the protein

5. (...)

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thank you sir

Hi Ghaz,

 

unfortunately there are many reasons that can justify the lack of activity of an eukaryotic protein produced by bacteria. Some of the most recurrent problems are:

1. Absence of post-translational modifications in the producing cell

2. Absence of chaperones suitable for a proper folding of the protein

3. Non-specific interactions with host proteins and/or other molecules

4. Structure alterations due to lab manipulations during the isolation and purification of the protein

5. (...)

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Share on other sites

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