Jump to content

Recommended Posts

I work on differentiation of stem cells to chondrocytes. I used pellet culture system for that.

now I want to do gag assay.

but I do not know how to digest this pellet and then use papain digestion solution.

and I have a another question about which concentrate of papain in suitable for gag assay.

thanks .

Azadeh

Link to comment
Share on other sites

  • 5 months later...

Hi Azzadeh,

 

I am doing my master's thesis within few days. I also did GAG assay as a marker for chondrogenic differentiation, but the problem is, I cannot find anything to support such claim. Could you please mention anything about how is GAG assay a marker for chondrogenic expression ?

 

Thanks in advance, and looking forward to hear from you and perhaps share my experience in this regard.

 

Greets

G

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
 Share

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.