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I work on differentiation of stem cells to chondrocytes. I used pellet culture system for that.

now I want to do gag assay.

but I do not know how to digest this pellet and then use papain digestion solution.

and I have a another question about which concentrate of papain in suitable for gag assay.

thanks .


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  • 5 months later...

Hi Azzadeh,


I am doing my master's thesis within few days. I also did GAG assay as a marker for chondrogenic differentiation, but the problem is, I cannot find anything to support such claim. Could you please mention anything about how is GAG assay a marker for chondrogenic expression ?


Thanks in advance, and looking forward to hear from you and perhaps share my experience in this regard.




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