apricimo Posted April 13, 2011 Share Posted April 13, 2011 Looking to coexpress two vectors pCDFDuet and pETDuet in E. coli. Origins of replication are CDF and F1 respectively. Anyone know if these are compatible? Yes they are compatible Link to comment Share on other sites More sharing options...
CharonY Posted April 16, 2011 Share Posted April 16, 2011 IIRC the duet vectors are routinely used for multiple co-expression, so as long as you use a host strain that is compatible to both plasmids, you shold be fine. Novagen also sold at least one of those. Novablue or something like that. Other strains include B834 and/or Bl21 derivatives, I think. But better check that out, as my recollection is a bit hazy on that. Link to comment Share on other sites More sharing options...
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