hodderhansen Posted March 24, 2011 Share Posted March 24, 2011 I was just wondering if anyone knows the formula to convert an absorbence reading from a spectrometer reading to the amount of protein in micrograms. Link to comment Share on other sites More sharing options...
Greippi Posted March 25, 2011 Share Posted March 25, 2011 (edited) You need to create a standard curve with the absorbance readings of known concentrations of protein. You can then read the absorbance of your unknown and read off the concentration from your graph. ALTERNATIVELY A280 (1mg ml-1) = (5960nTrp + 1280nTyr + 120nCys) / Mr where nTrp, nTyr and nCys are the numbers of tryptophan, tyrosine and cysteine residues respectively, and Mr is the predicted molecular weight of the protein Edited March 25, 2011 by Greippi Link to comment Share on other sites More sharing options...
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