soconfused Posted September 25, 2004 Share Posted September 25, 2004 chromatography: we have to collect the fractions from the column. i wonder what is the difference between; flowthrough fraction, wash fraction and eluate fraction? i think i know what it means with eluate fraction, but what about the other two? hope for replies! thanks a bunch! Link to comment Share on other sites More sharing options...
Frostrunner Posted September 28, 2004 Share Posted September 28, 2004 flowthrough= the liquid that went out of your column while the substance of interest stayed bound to the column when you applied the starting liquid mixture normally after binding you wash a column to get rid of immurities wash fraction= the liquid that came out of the column as you were washing it. after washing you apply a elution buffer eluding fraction= what liquid came off the column as a result of the eluding buffer. this should have you substance of interest. You are probably collecting the different fractions to see if you lost alot of protein/dna/substance when you washed by testing wash fraction and to see if the column worked at all by testing flowthrough fraction. Link to comment Share on other sites More sharing options...
soconfused Posted October 2, 2004 Author Share Posted October 2, 2004 thanks very much for your sharing, Frostrunner! Link to comment Share on other sites More sharing options...
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