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how to separate membrane from cytoplasms in diatom

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Hi folks,


I have some diatoms in culture and I am looking for literature about lysis and subsequent separation of cell components (membrane from cytoplasmatic constituents). I was wondering if anyone here has worked on that topic, or even if you have any tips.



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Oddly enough, I did this yesterday.


But I did it on bacterial cells, maybe it's different for diatoms.


I French pressed my cells to lyse them. Then I applied the stuff to the top of a sucrose gradient. Ultracentrifuge over night, then harvested the membrane band.


I can give you a more detailed protocol if you like - but the conditions will probably be different for diatoms. It also depends on what you intend to do with the membranes.

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