2mg/ml from a 2U/ml solution?

[question]

hello guys!

 

I need to prepare a 2 mg/mL DNAase solution. the DNAase stock has a concentration of 2U/ml. how can i make a 2mg/ml from a 2U/ml solution? this is quite hard to figure out

 

 

 

hope very much for replies!

 

thanks for helping!

[Skye]

Are you sure you don't have to produce a 2 mg/ml solution of the substrate?

[question]

hi skye!

 

what do you mean? the DNAase i bought was quantified in Units and i have to make a 2mg/ml solution from that stock. hope that you and the others can help me out!

 

thanks alot!

[badchad]

First I think you have to convert the stock solution of "units" into weight. Right now it seems to me that your attempting to convert two completely different things. Once you can convert the stock soultion to weight, it is quite easy, use the old equation:

 

(C1)(V1)=(C2)(V2)

 

(Concentration of the first solution)(Volume of first solution)=(concentration of second solution)(volume of second solution).

 

just remember V2 is the final volume. Now you already know what C2 is: (2mg/ml). Decide how much of that you need, which would be V2.

 

Once you determine the conc. of the stock (C1), you'll have one variable left. solve for x. (in this case V1).

[Skye]

I was hoping you'd just made a simple mistake reading some instructions, but I guess not.

 

Did the enzyme you bought come with any data sheets?