cazmantis Posted June 30, 2010 Share Posted June 30, 2010 Hiya, I am currently BLASTING genomic data in FASTA format between species to try and identify a conserved locus. I have obtained quite a good list of BLAST results, but what I am specifically looking for are matches in the flanking regions of the gene to make sure they are a product of the same locus. I have been looking into this for a week or so and my progress is...slow to say the least! I can blast the gene which specific mRNA of interest is derived from (I am using genomic DNA), and of course once I obtain a result I can see where the matches are in the nucleotide sequences. I am just really stuck at identifying where in the piece of genomic DNA I'm looking at the flanking region is! I am aware that it will be at the 5' end and will contain elements such as signal peptide and TATA box, but my lack of experience is really holding me back on figuring this out. Could anyone help me out with any pointers? Thanks so much! Caz Link to comment Share on other sites More sharing options...
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