A plasmid problem

[dttom]

I met a problem in manipulating plasmid in my project, here it goes.

 

I have a plasmid with an insert, which is capped between two identical promoters in different directions, so a dsRNAs could be produced. Now if I want to chop the insert into pieces, then just incorporate one piece (a random piece) of them in bewteen the pair of 'caps', what should I do?

 

Any comment would be appreciated, thanks.

[CharonY]

There are various options, depending on how you inserted the insert to begin with (and what kind of vector it is).

 

Do the promoters have convenient MCS or are they part of the insert?

[dttom]

The plasmid don't have a MCS but you reminded me to look at the detail structures of it and the problem has been solved now, thank you.