littlemok Posted September 19, 2007 Share Posted September 19, 2007 Hello, I was wondering if anyone could help advise me. I am trying to do enzyme inhibition kinetics but according to protocols I need to do it using the substrate to zero the spec with first and then look for a decrease at a certain wavelength. When this happens there is a decrease but the absorbance goes negative, consequently lineweaver burk plots face downwards. I do not know enough about this as this is the first time I have ever tried an enzyme assay, Can anyone suggest how I could change things? Many thanks to anyone who replies Link to comment Share on other sites More sharing options...
Bluenoise Posted September 21, 2007 Share Posted September 21, 2007 Could you tell us something about the reaction? Link to comment Share on other sites More sharing options...
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