Bluenoise Posted August 10, 2007 Share Posted August 10, 2007 Okay, lets say you isolated a bacterial mRNA through the binding of another RNA of much smaller size (say 70bp) how would you go about cloning and sequencing this bacterial mRNA you isolated? Rules: 1) Bacterial RNA does not have a poly A tail. 2) You're completely clueless about the sequence of this mRNA you've isolated. 3) Your sample is contaminated by this small RNA by thousands if not millions of times the copy number of the RNA you're trying to isolate. 4) There are thousands of possible binding partners for this small RNA so you can't just search for the anti-sense. So you basically have to selectively clone this mRNA and sequence it. I've thought of a possible way to do it. But I want to hear someone else's suggestion first. Link to comment Share on other sites More sharing options...
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