CM Posted March 16, 2007 Share Posted March 16, 2007 Hi, I'm looking for the "best" method to separate lipid vesicles and unbound protein. The situation is as follows: Some protein gets reconstituted on the vesicle, but, of course, some residual protein won't reconstitute (reconstitution in this case has an efficiency of ~80%). Now I need to remove the unbound protein (a monomer of ~35 kDa) from the solution without diluting it too much. Any hints / recommendations? TIA Christian Link to comment Share on other sites More sharing options...
Bluenoise Posted March 18, 2007 Share Posted March 18, 2007 dialysis? Link to comment Share on other sites More sharing options...
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