Lola Vargas Posted February 16, 2007 Share Posted February 16, 2007 Hi, I have a question regarding antibody concentration in immunized rats. When immunizing them with a recombinant protein, what's the "expected" concentration of IgG to my protein? I understand this must differ a lot (!), but does anyone have a feeling for what a reasonable concentration range might be? Are we talking mg/ml, ug/ml, ng/ml? What's the avarage concentration of total IgG in a rat? 3-20mg/ml? Cheers, Link to comment Share on other sites More sharing options...
Lola Vargas Posted March 4, 2007 Author Share Posted March 4, 2007 Doesn't ANYONE know this?!? Very surprising.... Totally useless... Link to comment Share on other sites More sharing options...
badchad Posted March 4, 2007 Share Posted March 4, 2007 If you're using a recombinant protein, the amount of IgG formed will be relative to the immune response generated against the protein. Theoretically, a benign protein wouldn't generate much of a response and you could inject tons of protein into your rat and get nothing. As you stated, there is absolutely no way to tell. I suggest looking at some previously published literature. Link to comment Share on other sites More sharing options...
JackMuChabas Posted August 17, 2007 Share Posted August 17, 2007 Yes there is a way to tell by using the gluco protein response and surmising it level of deterrence. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now