KFC Posted August 19, 2006 Share Posted August 19, 2006 I've isolated some DNA in ethanol(70% Solution) from Split Pees is there a way to separate ß-2-Deoxy-D-ribose, Thymine, Adenine, Guanine, Cytosine, TMP, AMP, GMP, or CMP. Link to comment Share on other sites More sharing options...
woelen Posted August 19, 2006 Share Posted August 19, 2006 How are you sure you isolated DNA? Don't you have any other organic crap from the cells, other organic matter, etc. ? It seems quite difficult to me to isolate DNA. Link to comment Share on other sites More sharing options...
KFC Posted August 19, 2006 Author Share Posted August 19, 2006 http://learn.genetics.utah.edu/units/activities/extraction/ http://biology.about.com/c/ht/00/07/How_Extract_DNA_Human0962932481.htm Not sure if its pure it probably has some impurities like RNA or Enzymes or something. Link to comment Share on other sites More sharing options...
insane_alien Posted August 19, 2006 Share Posted August 19, 2006 you probably have a lot of impurities. Link to comment Share on other sites More sharing options...
woelen Posted August 19, 2006 Share Posted August 19, 2006 Using such a simple procedure definitely gives you very impure DNA. It will be hard to separate the DNA from the other organic stuff. Link to comment Share on other sites More sharing options...
Karnage Posted August 20, 2006 Share Posted August 20, 2006 why don't you use lab equipment to isolate DNA? This method you use is quite simple and doesn't yield a high percentage of pure DNA Link to comment Share on other sites More sharing options...
insane_alien Posted August 20, 2006 Share Posted August 20, 2006 maybe because he doesn't have lab equipment? Link to comment Share on other sites More sharing options...
John Cuthber Posted August 28, 2006 Share Posted August 28, 2006 I think that procedure probably gives reasonably clean nucleic acid- certainly if there were a lot of trash in it, it wouldn't be a stringy goo like DNA. I think it's not too dificult to hydrlyse DNA to its constituents. Enzymatic hydrolysis would be neatest but I think a strong base would do. Link to comment Share on other sites More sharing options...
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