beachbum Posted February 20, 2006 Share Posted February 20, 2006 Hi, i am doing a project on yeast. lipopolysaccharide is usually used in mammalian cells to induce NOS expression but does anyone have literature that has demonstrated yeast sensitivity to LPS? secondly, how does someone come upon identifiying a downstream regulator to a process? is it mainly just reviewing literature like determining an inducer/inhibitor for a process or more different? Thankyou. Link to comment Share on other sites More sharing options...
THE V Posted March 18, 2006 Share Posted March 18, 2006 Google scholor is really nice Link to comment Share on other sites More sharing options...
beachbum Posted March 18, 2006 Author Share Posted March 18, 2006 thanks but i've checked google scholar and the University of florida library database but nothing has come up about yeast being sensitive to LPS addition. Link to comment Share on other sites More sharing options...
zyncod Posted March 18, 2006 Share Posted March 18, 2006 I really doubt that yeast would be responsive to LPS - what would they have to gain from it? Actually the TLR responsible for LPS recognition also recognizes zymosan. Link to comment Share on other sites More sharing options...
beachbum Posted March 18, 2006 Author Share Posted March 18, 2006 yes thats true that yeast wouldn't have anything to gain from it but it could be just a stimulated response. the zymosan thing is pretty interesting, but I really can't risk using LPS if there isn't published support. I've checked chemical company catalogues and nothing has come up but by any chance, do you know of any NOS inducing drugs? Link to comment Share on other sites More sharing options...
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