Jump to content
Sign in to follow this  
PuelloDiablo

Expression cassettes

Recommended Posts

Hey guys,

 

Have a question: when making transgenic animals, you use an resistance cassette for screening the cells(e.g. Neo = G418 resistance), you make chimeras and mate them with wt.

I'm trying to understand an article I'm reading, where they remove the cassette by crossing the F1 generation with mice that has a special recombinase that will excise the cassette.

 

Why do you even need to remove resistance cassettes? Would it matter if you just kept it in the germ line?

Share this post


Link to post
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
Sign in to follow this  

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.