Defective 96-well, polypropylene 1 mL round well microplate

[Stack344]

Our lab is experiencing a major issue with PCR results. We switched to a new lot of 96-well plates and suddenly started seeing rampant false signals causing QC failures.

The specific product in question is the Agilent brand: Storage/reaction microplate, 96-well, polypropylene, 1 mL/round well, round bottoms, 32 mm height, irradiated. Part Number: 204357-100.

Looking to see if any other lab out there is or has experienced a similar problem, especially specifically with this equipment.

Edited January 6, 2022 by Stack344
Formatting

[CharonY]

55 minutes ago, Stack344 said:

Our lab is experiencing a major issue with PCR results. We switched to a new lot of 96-well plates and suddenly started seeing rampant false signals causing QC failures.

The specific product in question is the Agilent brand: Storage/reaction microplate, 96-well, polypropylene, 1 mL/round well, round bottoms, 32 mm height, irradiated. Part Number: 204357-100.

Looking to see if any other lab out there is or has experienced a similar problem, especially specifically with this equipment.

Are you using it for qpcr or pcr? Not using those plates but they do not seem be made for either application based on well shape and lack of wall thickness info.

[Stack344]

13 hours ago, CharonY said:

Are you using it for qpcr or pcr? Not using those plates but they do not seem be made for either application based on well shape and lack of wall thickness info.

We don't use them for the qPCR reaction itself. We use them for RNA extraction and various reagent storage purposes. The plates as a whole have never been an issue, just a particular lot.