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Nick 102

Cloning guide RNAs

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Hi Guys-

have a question , I’m trying to clone guide RNAs into a vector with Bfu1 sites. I was told to make an oligo that has

 a protospacer sequence- bfuSite/forward- reverse bfu1 site.

the protospacer is the same as the forward guide sequence without the Bfu1 site


i don’t understand what the protospacer is???

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