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txxplc110

HPLC peak resolution

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Hello! I have a question about HPLC chromatograms. I got two chromatograms, each one with different signal/wavelength (290nm and 325nm) and I got two peaks of compounds that I wanted to analyze, but the peaks are not on the same chromatogram. My question is, can I read peak resolution of two peaks from two different chromatograms, or not? It doesn’t make any sense for me, but it's in my instruction. I can’t find any information about that. I would be most grateful if you would look into this matter.

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