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Calvin P

How to transfer a gene into agrobacterium without having the gene being expressed by agrobacterium

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Hello everyone I have a genetics related question on how to achieve a transformation in agrobacterium without having the gene being expressed bu the agrobacterium.  According to what I have learned it seems relatively simple to achieve a transient transfection with plasmid vectors and cationic liposomes in bacteria.  However my concern is that this approach will result in the  agrobacterium expressing the gene instead of adding the gene to it's Ti plasmid.

The goal is to insert a firefly luciferase gene into Arabidopsis.

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