BabcockHall Posted October 26, 2019 Share Posted October 26, 2019 Ordinarily the polyacrylamide gels are rubbery, but the ones I poured last week were very stick, and the stacking gel did not seem to solidify. I was able to run a pre-stained standard, and it looked almost normal. However, the gel stuck strongly to the glass plates and was unusable. I made new acrylamide using a different manufacturer's bis-acrylamide, and the new gel behaved normally. Either it was the bis, or I made some other mistake in making the acrylamide solution. Has anyone ever seen something similar? Any guesses about what could cause a gel to become sticky and less of a solid? Link to comment Share on other sites More sharing options...
CharonY Posted October 28, 2019 Share Posted October 28, 2019 This is sounds like either incomplete polymerization or lack of cross-linking. This is often due to old APS or wrong acrylamide bis-acrylamide ratio. Link to comment Share on other sites More sharing options...
BabcockHall Posted October 28, 2019 Author Share Posted October 28, 2019 The APS was fresh, but the first bis that I used was old. There is also a chance that I made a gross error in weighing it out. Link to comment Share on other sites More sharing options...
CharonY Posted October 28, 2019 Share Posted October 28, 2019 Considering that you manage to resolve your marker, I suspect that you got mostly linear acrylamide. Measurement errors could account for that. If you used powder, bis-acrylamide is relatively stable, but I do not think that I had a batch lasting longer than a year or maybe two. Link to comment Share on other sites More sharing options...
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