Jump to content
Sign in to follow this  

SNP "Flip"/"Invert" and Sequencing

Recommended Posts

I'm working with raw SNP data from Embark (think 23&Me for dogs).

I looked up a genetic mutation in a university study, and some (not all) of the SNPs in the report are inverse (A<-->T and C<-->G) to the raw data from Embark. Is there a standard for this? If the study found T at a specific SNP, how do I know whether an A detected by Embark is a different variant, or simply the same base pair viewed from the other strand? I would think they could write a probe to get the same SNP from whichever strand they want, but then they would have to know which value correlates to each variant for their particular probe, wouldn't they? Would it be common for unrelated labs/tests to study the same base pair from opposite strands?

Also, I get heterozygous SNPs like AG then AG. But I presume there is no telling whether these peptides are actually sequenced AA and GG on the strands or AG GA, correct? Taking that a step further, it would appear to actually be impossible to actually sequence from SNPs simply because they do not distinguish strands. Is that correct?

Edited by cpurick

Share this post

Link to post
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
Sign in to follow this  

  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.