TeslaTurbine Posted April 9, 2019 Share Posted April 9, 2019 Hello everyone, I need to cap gRNA's. I've found a product online to do so. However it requires that the gRNA has a di- or triphosphate at its 5' end. I'm new to the CRISPR and RNA world in general. Do gRNA's have this di-/triphosphate at their 5' ends? I know this seems like a relatively easy question to find out, but I've been rummaging through literature for hours trying to find this one tiny bit of information and have come up with nothing. Link to comment Share on other sites More sharing options...
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