Sigma90 Posted February 16, 2018 Share Posted February 16, 2018 I would like to measure different enzyme activities in centrifuged pig kidney homogenate. I get out of range errors at 450 nm. The samples are still reddish after centrifugation. Is there a way to remove hemoglobin without deactivating / removing enzymes? Thanks! Link to comment Share on other sites More sharing options...
BabcockHall Posted February 18, 2018 Share Posted February 18, 2018 Do you want to measure the amount of each via protein quantitation, or do you want to measure the rate at which an enzyme catalyzes a particular reaction (often called its activity)? You may need to fractionate your sample with standard protein purification techniques. Link to comment Share on other sites More sharing options...
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