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Posts posted by Mirabelle

  1. I think a combination of the two above posts would be my advice. Sports keep your muscles tight and Pilates (or "Teh Rack") is designed to strengthen and stretch the muscle. And of course, the muscle is attached to the bone.

    I'm 5'12" (That's what I usually tell people) and I was in MMA for a few years, it's true that taller is NOT always an advantage but the long limbs can be.


  2. Electric cars run so quietly I don't hear them coming. That is very concerning to me (for the visually impared that rely on that sound for safety.)

    Maybe they could add little whistles like they used to have to prevent deer from running in front of you only slightly louder.

    My favorite car is 25 years old. It runs beautifully but is not environmentally friendly (the cost to make it so is beyond my capasity at the moment).

    I love it though and it's very special to me.

  3. top down: therm2.JPG


    Is that a compressed gas cylinder sitting in front of the wall vent? That’s not a heating vent, right? Also, I like the wall color is that paint or wallpaper?



    Anyway, I was looking at giant wall thermometers the other day and they are expensive! If you mounted that on a nice wood plate and change the color to a less pink hue, you could make good money.

  4. But Dak's got a point. I've known many people suffering from depression, and while they do feel hopeless as you say, if they didn't think someday they'd feel better then I'm convinced all of them would commit suicide - it's debilitating and the self loathing becomes a futile feedback loop. It's grim, and it's not clear to them, but it's in them and is the only reason they try. Obviously this doesn't apply to everyone, but I do believe it does most.


    I agree. Simply by seeking help for their depression shows they know somehow this can be better or they still have hope that it can.


    However, I do not feel that a person suffering through "clinical" depression has the clarity to request and be granted euthanasia.


    How many people who have been through depression have seriously thought about suicide and then after they recovered regretted even thinking it?


    I can not to see the choice of euthanasia as a "yes or no" for all persons in all situations.

  5. When I get to class, the professor jumps into his lecture and I tune him out. I read and digest the book at my pace, wait until the lecture is over, and head home.


    Have you tried reading the chapter prior to lecture so you have it already digested and may gain further insight from the lecture?

  6. don't most depressed people know that they're depressed, and that the feelings will either go away or become lesser over time with proper treatment, even tho they don't neccesarily feel as if that's the truth?

    Such clarity is not usually present in depression. Perhaps being in a foul mood you may realize that it will pass but in most cases of depression it's not a matter of "get over it" or "This too shall pass".


    There is a very often an accompanying feeling of true hopelessness and sometimes self loathing that could be what is leading them to consider suicide.

  7. Thank you


    I'm a bit of a pack rat.

    Every other year or so I buy one of those "pumpkin carving kits". I never use the designs but I always save the tools and each year they add a new tool so I've gathered a large collection (well organized too).


    This was done with a very small (1.5-2mm wide) saw blade.

    For me the hardest part is determining what parts to cut out first so the surface remains strong enough to be detailed without falling apart.

  8. A BS biology or biotech graduate should absolutely know sterile techniques, how to use the basic tools of the trade, and proper documentation. They also need a basic understanding of statistics. In my opinion, I believe that this (stats) is probably the most important thing most programs are missing..

    I am currently working to teach myself the variables that determine what is "statistically significant".

    There are bio-statistians that handle this for clinical data but it seems a valuable skill in design of process and analytical validation projects.


    "Too high"? How can you make this statement? Why would you settle for hiring people with these deficincies? As long as they are being hired, the programs won't change..

    I don't hire them, this fact only adds to my frustration. I usually give a list of suggestions/additional teaching to the AS biotech program each year after the students complete their internships with us.

    It may end up in the wastepaper basket unread but it’s all I have time to do right now.


    BTW: I agree that cGMP, cGTP, and clean room regulations and standards can be learned on the job......or in electrive programs...but htey would certainly be a positive factor in a hiring decision.

    I do understand that these are industry specific and would not normally be in a general biology curriculum.

    The cGMP, cGTP, and clean room standards are a major component of working with my/their current employer. Never the less, they maintain this attitude that these are minor issues and when they are brought to attention it is corrected and forgotten.

    I have suggested that these employees should have an audit of their work, skills and practices to get a better understanding. I have to do it in my lab, it’s unpleasant but if it is done properly it is a learning tool and not an act of torture.

    Any advice for training such aloof employees?

  9. Some of the recent B.S. (Biology and biotech) and a few M.S. graduates we have hired required being taught:

    -how to make a 1:10 dilution

    -serial dilutions

    -to manually calculate the viable cell count from a hemocytometer

    -significant digits/reporting results to scientific form (X.XXe+0X)

    -to make it a common practice to properly label the value (90.6+e08 µL...say what?)

    Not calculus, not statistics, just basic application is either absent from the curriculum or lacking.


    I do wonder if it is caused by the individual’s ability to recall the knowledge and not the quality of the education received.


    Besides math issues:

    -inability to properly use a serological pipette, pipet-aid, or other common instruments

    -aseptic techniques

    -importance of proper documentation

    -understanding of cGMP, cGTP, and clean room regulations and standards


    What makes it most frustrating for me is the attitude that these are minor issues and when they are brought to attention it is corrected (if possible) and forgotten.


    Has anyone else in industry seen this behavior or are my expectations too high?

  10. I worked at Argonne for 6 months as an undergraduate. The "government" has nothing to do with it!

    Just to clarify:




    The government may have nothing to do with the quality of the work produced but the laboratory is operated by UChicago Argonne, LLC for the U.S. Department of Energy's Office of Science.


    Now, back to ADULT stem cell technology...

  11. Very true!

    In fact the technology was bought without solid review of the data and independant testing. So when they handed us the "Procedure" and we followed it exactly we went from Monocytes to multi-nucleated osteocytes... Not good! But research was done and corrections were made.


    Here is one publication I had on hand:


    Zhao Y, Glesne D, Huberman E. A human peripheral blood monocyte-derived subset acts as pluripotent stem cells. Proc Natl Acad Sci U S A. 2003; 100: 2426-2431

  12. ARGH! I hate reading over my posts and seeing how poorly I stated thoughts.


    Let's try again:

    The company I work for is currently preparing an animal study for the treatment of type II diabetes. The developed technology can de-differentiate PBMCs and direct them into insulin secreting islet cells. In the pipeline is to take PBMCs to heart muscle.


    Better...but not much.

  13. Oh no...He was the reason I was going through withdrawls.

    Here is a (not-so) brief:

    He put me on the SNRIs, over the past 2 years he has changed his diagnosis at least 5 times and the medications even more often. He had me on 375mg Effexor XR, I spent a almost 2 weeks trying to get in contact with him to approve a refill, by the time his nurse returned my call I was already beyond withdrawl and recovering. I made an appointment to see him anyway and I went in and told him I feel better, he diagnosed SNRI DS. I told him I did not want anymore SNRIs. He told me to try Cymbalta, I explained to him that I was aware of Cymbalta and that I would like him to respect my request to NOT take another SNRI.

    He and I did not agree on many things.

    He once told me I should lower my morals and standards. I told him he needed to find some.

    Most doctors are NOT like this one but it is important to research any diagnosis or medication you receive and speak out if you think the doctor is wrong it will help both patient and doctor.

  14. I think that as cancer research goes on, we may find out more and more about how our cells work. For instance, with diabetes I've always wondered why they can't get the islet cells in the pancreas to re-grow. I wonder why they can't investigate the cells of pancreatic cancer to see if any of them are islet cells. Then they could figure out a way to 'turn on' those cells again so that they can re-grow, then turn them off so they aren't a 'cancer'.


    The company I work for is currently preparing an animal study for the treatment of type II diabetes. The developed technology can dedifferentiate PBMCs to insulin secreting islet cells. In the pipeline is to take the dedifferentiate PBMCs to heart muscle. On step closer...


    You do touch on a point I think is often over looked in the stem cell debate. Can they truely be controlled? Do people realize the the incredible potential of stem cells carries an equally incredible risk?

  15. I think a good therapist is very important.

    I do not engage in conversation comfortably nor am I able to "just talk" and I got a therapist who would NOT shut up. I left there feeling worse because she made assumptions about me and I wasn't able to defend myself. What a horrible match that was.

    It did make me realize how lucky I was to be able to keep trying different therapists until I found a really wonderful one. Some insurance companies/student services don't allow that.

    I find it oddly interesting that after dealing with the different medications "to help" and stopping without consulting my doctor (again this is a BAD idea), I got told by the prescribing doctor that I now have "SNRI discontinuation syndrome" and he prescribed Neurontin. I stood up and walked out on him.

    I believe a good doctor is important too.

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