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DeoxyriboNucleicAcid

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Posts posted by DeoxyriboNucleicAcid

  1. Your district must have cut the budget down. That can be a b*tch. It almost happened in my HS, but luckily it did not. Unfortunately, AP/ honors classes are not required for schools to offer by most states, so they can be easily droped because of money matter and/ or low acceptance rates/ class sizes. I suppose you don't have too many electives available either.

    Well, good luck next year.

  2. Thanks for your replies inamorata and Skye,

    I am thinking along the lines of electroporesis, maybe even a combo of heat shock as well. I will have to get these pores as big as possible, and quickly too, to produce suction. Ill look into those companies, that will solve my first problem of getting the full genome.

     

    However, the host cell must loose it's DNA to do the experiment.

     

    QUESTION:

    Are there any methods of neuralising DNA inside a cell. without killing it? If the DNA is neutralized, it wont make RNA, and Rhibosomes will have nothing to do. There is a lag time between Neutralizing, and the cell haveing nothing to do, correct?

    Maybe the DNA will be inserted within that time frame?

  3. Hi Everyone.

    (I do have questions, but want to give you my background info first.)

    I am planning on doing an experiment to make one species of bacteria into another.

    I plan to:

    Take E. coli bacteria and extract all of their DNA; their entire genome.

     

    Then, I will take another species of bacteria (strep) and remove ALL DNA from them.

     

    I shall next take the E. coli DNA and force it into the strep.

     

    I want to see if the strep cells will indeed turn into the E. coli bacteria.

    None of this is finalized. The bacteria species and types may vary if it is easier to do this experiment. The DNA may be cut with enzymes, or whatever, as long as it goes into the 2nd bacteria type.

    Efficiency rates are not a problem, as long as there is a small or some percent of chance of success.

     

    =====

     

    MY QUESTIONS:

     

    What are the lab methods of extracting a full "loop" of DNA from bacteria?

    Is there a way at all to extract all of the DNA in a cell (many cells will be used when I do this.)

     

    If the bacterial DNA is broken into many segments when extracting (if that is the only way,) will it reassemble with sticky ends inside the host (2nd) bacteria species.

     

    What are the methods of destroying/ taking out all of the DNA in a cell without killing that cell. Can it be done?

     

    What are the methods of forcing DNA into bacteria cells without it.

     

    Basically, this is like a clone, with no egg cell.

     

    ANY IDEAS/ RECOURCES WILL HELP ALOT.

    Has it been done before? Where? How can it be reproduced.

  4. Hello,
    I have recently found a number of weird rocks on a Long Island beach.
    The rocks appear to be iron based.
    They have red-brown currosion on them.
    They have a red-brown streak, and are very hard.
    Metallic Luster.
    No APPARENT cleavage, but then again, they look like they are in layers?

    I also found that they are highly attracted to a magnet. Even more interesting, I was able to find their polarity (+/-) using some magnets.


    Can someone please identify/ tell me more about this mineral/ rock???

  5. Well, its antennae are there, just hanging down in the wrong direction. I couldn't perk them up:) The "Japanese" beetle is about 3/4 inch long.

    The others are about 1.5 to 2 (im just estimating.)

     

    HOWEVER: Through many many many many google searches, a few somewhat comprehensive dichotomous keys and an Autobun guide, I have successfully identified all 4 specimens.

     

    The First: Longhorn beetle; Fam: Cerambycidae, Genus: Archodontes, Sp: melanopus.

     

    The Second: Same species as first; just different age.

     

    The Third: Ground Beetle: Fam: carabidae, Genus: calosoma, sp: sycophanta.

     

    The ground beetle is really a beutiful specimine, he is in perfect condition, and is full of irridecent colors. Greens, oranges, blues, etc. He was a nice find.

     

    The Fourth: Scarab Beetle: Fam: Scarabaeidae, Genus: gymnetis.

     

    Sorry for terrible grammar, I’m on the rush today.

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