Ben99

Hello, 

I have some doubts about genetics that I had during my studies. 

Considering the sequence ACGCGT, what does it indicate? A splicing site, a restriction site? 

The other part is: 

If I have the virus B. Subtilis, what is true about the transcription of his genes? 

I did not understand if it uses or not the polymerase of bacteria and If it uses or not sigma70 for transcription of early genes.

Also what it is not really clear to me is if it uses or not sigma of bacteria and if it uses or not viral sigmas to transcribe intermediate genes. 

I know that are maybe difficult but I am pretty confused.

About the question related to ACGCGT I suppose is a splicing site but I am not really sure, about the other topic I am really a 0. Thank youu

1 minute ago, Dagl1 said:

Probably not, epigenetic modifications can induce transcriptional changes, but if a tumor has a mutation in a gene necessary for its survival, then epigenetic modifications will not help to get around that mutation. Of course, you could alter some characteristics of the tumor (but it will probably be changing those back), but you cannot bring it back to an healthy condition without changing the genome as far as I can think of. 

How one would do that is even more difficult, you would have to induce methylation and histone modifications at many many sites, additonally you might want to change the location of the DNA within the nucleus to be closer or further away from the nuclear lamina. Some of these things you could do with very targeted dCas9 systems, which recruit factors. But then you would have to provide possibly hundred if not thousands of sgRNAs, and the question is if that would work. At that point it might be easier to 'just' try to find and edit away every mutation.

Maybe other people can think of some ways that you can do this, but I myself can't come up with any way. 

I just edited my question because it was not complete, my bad:(

Anyway thank you for your answer. 

Hello,

Few days ago I had a doubt; can we use epigenetic modifications on a tumor cell in order to bring it back to a healthy condition? ( so acting only on the epigenome and not varying the genome).

On the other hand can we also use epigenetic modification on a healthy cell to transform it into a tumor one? ( is like the reverse of the first question above this one ) 

how can we perform both of the possible scenarios shown above (if it is possible to do these transformations ) 

1 hour ago, Strange said:

Is that possible?

(I suppose they could have been wrongly classified as different species, but then the genetic analysis shows they are actually the same.)

This question was asked in a test I found. They start from the fact that those 2 species of yeast have the same genome ( so It is something not debatable) and from that I have to say if they have or not the same number of genes and chromosomes, also motivate the Answer. The problem is that I do not know how to respond cause I think the question is a bit trick

The point is that the question is based on the concept that 2 species of yeasts are different but they have the same genome. How can I motivate if they have or not have the same number of chromosomes or genes?

Hello there, 

I have this question that I can not surely answer. For me the Answer is no but I am not sure and I can not motivate. Can Someone help me trying to motive the answer? Thank you 

Kind regards