Vexen

5 hours ago, studiot said:

Now would be the time to tell us what your favourites are.

2.The Blank Slate: The Modern Denial of Human Nature

---

3.The Better Angels of Our Nature

4.Freakonomics

5. 

Thinking, Fast and Slow

2011

On 3/6/2019 at 10:12 PM, peterwlocke said:

well i am not around people like this irl.

Why not?

6 minutes ago, QuantumT said:

Only nobody knows.

Can you explain further?

4 minutes ago, QuantumT said:

Probably because of the Higgs Boson.

Can you explain further?

What makes Scientology teachings false?

Was there anything before the big bang?

11 minutes ago, iNow said:

Where?

Is the laws of nature God?

I read somewhere that nothing is unstable.

What's the half life of dark matter?

What are your favorite popular science books?

I'm less confrontational in real life.

6 minutes ago, iNow said:

Breathing while you were temporarily banned

That's not a serious answer.

I wasn't temporarily banned. I just didn't post any topics. 

My progress is underwhelming.

What achievements are you proud of in your life?

This is just a rough overview of the DNA isolation of E. coli:
Centrifuge
Discard supernatant
Add lysis buffer
Add phenol
Spin 
A white layer should appear at the bottom and an aqueous upper layer and a middle phenol layer
Remove the upper aqueous layer and add to tube
To remove phenol add equal volume chloroform 
Spin and remove aqueous layer to new tube
Now you have to precipitate the Dna
Add ethanol to precipitate Dna
Spin
See the Dna pellet and discard the supernatant
Rinse in 70% ethanol
Resuspend in TE buffer (Add RNase to digest RNA)
Check on agrose gell

(https://bio-protocol.org/bio101/e97)

Dna absorbs maximally at a wavelength at 260nm. Nucleic acids absorbs UV light because of the heterocyclic rings. A study found "the level of fragmentation did not influence the accuracy of spectrophotometric measurements of DNA concentration".

Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods

Tatiana Sedlackova,^1 Gabriela Repiska,¹ Peter Celec,^1,3 Tomas Szemes,^2,3 andGabriel Minarik^1,3

When cell encounter dsDNA they presume it viral based and degrade it through RNAi. The Dicer enzyme cleaves short hairpin RNAs (shRNAs) into siRNA (single stranded RNA). The RISC complex binds the siRNA and guide it to complementary mRNA strand where it gets cleaved and eventually degraded. This is how cells degrades viral dsRNA.

RNAi will still work for multiple viruses invading a single host cell since multiple dicer proteins recognizes dsDNA.

^ Lee, Young Sik; Nakahara, Kenji; Pham, John W; Kim, Kevin; He, Zhengying; Sontheimer, Erik J; Carthew, Richard W (April 2004). "Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways". Cell . 117 (1): 69–81

1 minute ago, iNow said:

A better analogy is that religious people think of the soul like the tooth fairy, or santa claus since, while you assert it's effects can be seen, they actually cannot (whereas dark matter very much can).

Okay, That's a bit better.

Religious people think of the soul like dark matter. It hasn't been directly observed but its effects can be seen like consciousness and near death experience.

19 hours ago, Strange said:

I don't think that is the purpose of moderation. We enforce the rules and clean up the mess left by those who won't follow the rules. Kind of a cross between border patrol and trash collectors.

Lol, keep up the good work.

6 minutes ago, Phi for All said:

We would first suspend you. If you didn't get the message and continued in this vein, we'd suspend you for a longer period. If that didn't work but you weren't breaking any other rules, we'd probably put you in the moderation queue, where a staff member has to approve your posts before they show up for everyone. 

We'd rather you stop making this all about you, and just join some science discussions. Talk with people about the science, and stop trying to yank us all over onto your tracks. If you're serious about improving your life by improving your knowledge, it's not going to happen if you keep making your time here about everything else.

So, I shouldn't ask too many questions and only ask science and philosophy related questions in small portions and add substance to the questions?

Does your online personality correlate to your real life personality

32 minutes ago, zapatos said:

This is a science forum primarily for discussions regarding science and other topics of interest. You are treating this place like a chatroom for adolescent boys. You have been given a lot of leeway as you are new here, but I'm afraid if you don't get in the swing of things, you won't be long for this site.

I'm not new here. I've been here since 2014. Are you going to ban me for asking too many and treating this forum like a chatroom? I don't think I'm that bad.

1 hour ago, Arete said:

http://lmgtfy.com/?q=black+holes

You asked for advice to improve your somewhat meaningless internet reputation score and now you're arguing with that advice... that's probably not going to help. 

My questions aren't that easy to answer.

24 minutes ago, zapatos said:

Science does not support the concept of a religiously based soul. This forum is populated by people of science. Therefore you will not find many people here to defend the concept of a soul. That should be obvious.

Does that mean the religions based on the concept of a soul are invalid?