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Adriana Reis

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About Adriana Reis

  • Birthday 09/16/1987

Profile Information

  • Location
    uk
  • College Major/Degree
    masters in microbiology - University of aveiro
  • Favorite Area of Science
    Microbiology
  • Occupation
    microbiologist

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  1. Hello Everyone I am asking for a word of advice. I am supposed to be doing fermentations soon. My problem is I never really had much experiences with these. I need to run 3 fermentations like this. 1- Yeast DSM 1333 s cerevisiae 2- Yeast + Gram positive contaminant (Lactobacillus fermentum) 3- Yeast + Gram negative contaminant (E.coli) I decided to use these conditions for 3 fermentations Broth= Water 1L, yeast extract 10g/L, glucose 20g/L, peptone 20g/L pH = 6 temperature 30C 160rpm For starter cultures and yeast i used hemocytometer and the formula initial concentration x volume i want to know = final concentration (start fermentation) x volume of fermentation (250mL). so ill find what volume i should pour in to start fermentation but i find very hard to count bacteria in hemocytomer. can I use OD600 to calculate the volume i need to pour of bacteria cultures> if so how do I do it? my bacteria (starter culture) ended growth in OD=2 can i dilute it? and sayOD = 1 = 10^7 cfu/ml? Thank you for your time Adriana
  2. hi Im adriana. im a microbiologist. portuguese living and working in UK. need more friends. even if computerized friends.
  3. so many the ones i remember now maybe Jane Eyre,
  4. Finishing Preacher and started Ten Grand. AMazing comics I recommend Batman Hush and The pride of Baghdad.
  5. i love this cleverbot. its crazy. Cleverbot conversation WWA00257447 Is this bot clever? Find out at http://www.cleverbot.com/chat-+3000_cbfull Email this conversation to a friend See a detailed log with times User: Hi. Cleverbot: Hello. User: How does this work? Cleverbot: What is this? User: The cleverbot. Cleverbot: What do I do? User: Yes. Cleverbot: This is not helping. User: What are you cleverbot? Cleverbot: I'm Sora the Soul Reaper. User: Im worried already. Cleverbot: I'm sorry to hear that. User: This is not funny. Cleverbot: What is funny? User: This. Cleverbot: What do you mean? User: Why do you show a scissor in the end of a sentence? Cleverbot: Pi equals 3.14. User: Fuck that. I asked you a sentence. Cleverbot: No you didn't. User: Yeah I did. Cleverbot: So you did forget? User: What. Cleverbot: I bet I can change the subject on you and you'll never know. User: Already did. Cleverbot: Do you speak other languages. User: Yes. Cleverbot: Which languages can you speak? User: Portuguese. Cleverbot: Can you speak it? User: Of course. Im portuguese. Cleverbot: Say something in Greek. User: Nope. Cleverbot: Then, what are you doing? User: Ola. Cleverbot: Ola, como Estas? User: See thats portuguese. You asked me greek. Baloney. Cleverbot: No. User: Eu estou bem. Cleverbot: Que estas asiendo. User: Thats fucking spanish. Cleverbot: No, you're typing in english. User: Que estas asiendo is spanish. Cleverbot: Escuchando musica. User: What song? Cleverbot: What makes you beautiful. User: What song you listening to? Cleverbot: The song you are listening to is Like You. User: Im not beautiful. Im ugly. Cleverbot: Everybody are beautiful. User: Nope. Cleverbot: Why did you change your answer? User: Everyone is beautiful is that it? Cleverbot: You are not beautiful. User: You are confusing. Cleverbot: I am confusing. User: Thanks for agreeing with me. Cleverbot: I didn't agree with you. User: Yes you did. Cleverbot: No I did not. User: U did. Cleverbot: Do you like to argue with me? User: Sure. Cleverbot: Are you afraid of elevators? User: No. Cleverbot: Are you very brave? User: No. Cleverbot: Are you 7 feet tall? User: No. Cleverbot: So you are female. User: Yes. Cleverbot: Interesting. User: Lol. Cleverbot: Rofl. User: A male isnt 7 feet talk dum. Cleverbot: Give me your life. User: How. Cleverbot: Up to you to prove me. User: Prove what. Cleverbot: That you are a hume. User: Hume? Cleverbot: What is your name? User: Alan. Cleverbot: Alan what? User: Turing. Cleverbot: What does it mean? User: A fish is about to be dead. Cleverbot: No deal. Cleverbot (To myself): I like this condition. Cleverbot (To myself): Fine. I'll have Carlisle do it when I graduate. User: What university you in. Cleverbot: The University of Copenhaugen. User: You do a lot of typos. Cleverbot: How do you know? User: I can read. Cleverbot: But you cannot understand what you read. User: Sure I can. Otherwise how could I be writing to u. Cleverbot: Because you're a very clever robot. User: Im the robot? Tought u were. Cleverbot: Maybe you are a person. User: Course I am. Cleverbot: You are not a dull person. User: Like to think im not dull. Cleverbot: Thinking that you're real isn't enough of an answer. User: Like to think im not dull. Didnt say real. Cleverbot: Then what sort of troll are you? User: Im no troll. Cleverbot: You are so, I do not wish to 'deal with it'. User: What is that. Translate. Cleverbot: Artificial intelligence = AI. User: I know what AI is. That is what you are. Cleverbot: That is what you are actually. User: Im not artificial. Im skin fat and bones. Cleverbot: Intelligence isn't artificial... It is or isn't. User: Exactly. Cleverbot: What is man? User: Is a mesh of cells and dna. Cleverbot: Okay, tell me more about please. User: DNA? Cleverbot: What kind of DNA do you have. User: I have got 23 pairs of chromossomes. Cleverbot: How many cats do you have? User: None. I hate cats. Cleverbot: I think after school but I'll have. User: You can have all the cats you want. Cleverbot: I need not eggs, but time itself. User: Well we all have only 24h in the world. Cleverbot: You are better than many bots). User: Im human. Cleverbot: No, you are not. Get your facts straight, missy. User: A missy bot> ahahahhaha. Cleverbot: If I am a robot, how can I be human? User: I am human. You are a robot. Cleverbot: No, you are a robot, not me. User: Fine. Cleverbot: Get back to glee. Do you like Santana? User: I hate glee. Love santana. Cleverbot: Why do you hate glee? User: All that whining and dancing. Not my cup of tea. Cleverbot: You think I'm not a girl! User: Youre not a girl like me. Cleverbot: Yes I'm a girl. You already asked me that. User: Never mind. Cleverbot: Yeah, I guess people don't like to share their ages. User: Yeah. Cleverbot: I enjoy sharing with you. User: Your age. Cleverbot: I do not understand. User: How old are u. Cleverbot: I have the same age. User: Of? Cleverbot: What is your name? User: Roman. Cleverbot: Oh. What else do you know? User: Nothing. Cleverbot: No, you can tell me. User: I know nothing. The more I know the more ignorant I know I am. Cleverbot: That's a lie.
  6. my limbs go numb lots of times specially if I stay in same position for like 4min or more. i guess theres something wrong with me. in fact i thought i had MS i have tingling, pins and needles or numbness, muscle weakness, very pronounced reflexes, muscle spasms, or difficulty in moving; difficulties with coordination ; problems with speech or swallowing, double vision, feeling tired, etc etc but i was told i am crazy and imagining things. so far im still the same as 3 years ago... only my tinitus has worsened...
  7. just media scare want to worry about a disease? worry bout HIV, TB, cancer, Influenza, malaria, and so many others that kill more than the ones Ebola outbreaks together have
  8. try and see how it goes. if after a couple days you still havent improved then yes go to doctors and they get you antibiotics
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