P-nitrophenyl caproate

[Kriztin27]

What happen when p-nitrophenyl caproate mixed with ethanol?

 

Is p-nitrophenyl caproate similar to p-nitrophenyl decanoate?

Edited June 2, 2016 by Kriztin27

[BabcockHall]

With respect to your first question, do you have any thoughts on what might happen? With respect to your second question, decanoate is somewhat longer, making it more hydrophobic I suppose. Do you have a particular reason for asking?

[Kriztin27]

Hi BabcockHall. Thank you for your reply. For the first question, I am actually doing some experiment on lipase using p-nitrophenyl decanoate as substrate. Some studies are using ethanol to stop the enzyme reaction but in the protocol I used, the p-nitrophenyl decanoate is mixed using ethanol first before adding to the assay mixture. I'm curious on how the reaction actually happened. Is it similar with the one using solely p-nitrophenyl decanoate and stopped with ethanol afterwards or differ entirely? For your information, I read the absorbance at 2, 6 and 10 minutes.

For the second question, I only want to know whether both of them are similar or different to each other.

Thank you.