Jump to content

MIC values out of range


Silvia_84

Recommended Posts

Hi all,

 

please I really need your help. I am doing some MIC with Amikacin and the values I obtained so far for the reference controls are totally out of range and higher than expected. Can you tell me if the procedure I am following for the antibiotic diluition is correct? Just to be sure that it does not depend on me.

 

I prepare an amikacin stock solution of 5 mg in 500 ul.

I prepare my antibiotic dilutions that are in the range : 128-0.125 ug/ml. The final volume in each eppendorf (I have one for each antibiotic dilution) should be 1.4 ml and the dilution should be made two-fold . So what I do is to prepare the solution of antibiotic most concentrated (128 ug/ml) with this calculation:

I apply the formula CiVi=CfVf

 

Initial volume to be taken from the stock solution is = 128 x 1.4/ 5000/500 .This is 17.9 ul of stock

So the Volume of diluent is 1400 ul- 17.9 ul= 1382ul

From this dilution I take 700 ul and I pour it in the lower dilution (64 ug/ml) where already 700 ul of diluent are. And so on to halve the concentration everytime.

Is it correct?

 

Thank you very much in advance.

 

Silvia

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.