docjo.chua Posted June 7, 2015 Share Posted June 7, 2015 I need help with my thesis. Is there anyone here who studied EHEC in their school who happens to isolate it using Sorbitol MacConkey Agar? I am currently doing this study.. Link to comment Share on other sites More sharing options...
Micro.Pete Posted June 7, 2015 Share Posted June 7, 2015 Hi there, What is your question exactly? - EHEC differs from other E. coli strains being unable to ferment sorbitol, thus it uses peptone in the agar to grow and make colonies. The use of peptone raises the pH in the medium,. So EHEC will grow on MacConkey sorbitol agar producing pale to yellow colonies. Link to comment Share on other sites More sharing options...
docjo.chua Posted June 8, 2015 Author Share Posted June 8, 2015 Thanks Micro.Pete for your reply. I am currently studying EHEC O157:H7 from feces of native chicken. Do you have any journals or literature with isolation of the same organism from Chickens? Do you have any picture of EHEC O157:H7 colonies grown on SMAC which you can share? I can't seem to find any decent picture from the internet which may clarify what it supposed to look like. Thanks a Lot.. Here is my email docjo.chua@gmail.com Sncerely your,Jonathan Chua Link to comment Share on other sites More sharing options...
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