newbie17425 Posted June 6, 2015 Share Posted June 6, 2015 Hi, I got a PCR efficiency for all my targets and samples as 1. I did a quantitation analysis but it still calculated it as 1. I know that the R^2 value should be 0.9933 and slope should be -3.32 then by formula we get 1. But as i didn't run a standard curve, how reliable is this efficiency result? (lot of questions today). Thanks Link to comment Share on other sites More sharing options...
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