Jump to content

IPTG that is free of dioxane


Recommended Posts

It is hard to say, as cheaper batches may contain quite a varying degree of dioxane. It also depends on what you want to do. For standard induction assays to screen blue-white screening in E. coli (for example), it tends not to be a huge issue. It is rare that a batch contains enough (after the usual dilution) to kill them off completely.

 

However, if you want to use IPTG to induce something and then do some molecular analyses on the same strain (e.g. transcriptome or proteome analyses) the varying amount of dioxane can skew the results significantly. There may also be mutants or specific species that are more susceptible, but I do not have data on that.

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.