jvdb Posted May 23, 2014 Share Posted May 23, 2014 Dear all, I have a question about the use of mutant backgrounds in Arabidopsis. Just to confirm my thoughts. Mutant backgrounds are used to search for even more aberrant phenotypes. New i wondered, if you insert an extra mutation by for instance EMS mutagenesis in a certain mutant background, and you self your plants (because you want your newly inserted mutation to become homozygous), does your original mutated background remain homozygous? I thought it did, but i started to doubt.. Thx in advance! Link to comment Share on other sites More sharing options...
chadn737 Posted May 23, 2014 Share Posted May 23, 2014 If you start with a homozygous mutant before doing the second mutagenesis, then yes. Link to comment Share on other sites More sharing options...
taimoor Posted June 23, 2014 Share Posted June 23, 2014 if when a hetrozygous then? Link to comment Share on other sites More sharing options...
chadn737 Posted June 23, 2014 Share Posted June 23, 2014 if when a hetrozygous then? Then do the calculations. If your plant is heterozygous and you self it, 1/4 will be homozygous for the first mutation. It all depends on what generation of your mutagensis you are talking about. Are you working with the M1 or the M2? Link to comment Share on other sites More sharing options...
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